CRISPR

CRISPR Cas9 explained. CRISPR (clustered regularly interspaced short palindromic repeats) are segments of prokaryotic DNA containing short repetitions of base sequences. is an RNA-guided gene-editing platform that makes use of a bacterially derived protein (Cas9) and a synthetic guide RNA to introduce a double strand break at a specific location within the genome.

Cas9 is an enzyme that snips DNA, and CRISPR is a collection of DNA sequences that tells Cas9 exactly where to snip.

Reshaping evolution

January 10, 2022
TED Radio Hour | NPR

Illustration of DNA and parade of animals and man

Illustration of DNA with parade of animals and humans. (Adobestock)

New innovations in gene and stem cell technology have the power to shape ecosystems and even change humanity. This hour, TED speakers share the breakthroughs heralding the next scientific revolution.

Guests...

Genetically modified rice could emit fewer greenhouse gases

January 3, 2022
Eric Roston | Bloomberg

Rice planting

Photo: Worker planting rice. (Adobe Stock)

Fifteen years after their initial meeting to discuss what has become the CRISPR revolution, Professors Jill Banfield and Jennifer Doudna of UC Berkeley,...

Newly-discovered 'Borg' DNA Is unlike anything scientists have ever seen

July 15, 2021
Becky Ferreira | Vice.com

DNA strand

Image: KTSDESIGN/ Science Photot Library via Getty Images. Photo courtesy vice.com.

"Borgs" are extrachromosomal elements, meaning that these DNA sequences are found outside the chromosomes that lie within the nucleus of most cells and that contain the majority of an organism’s genetic material. Examples of extrachromosomal elements include plasmids, which can...

FDA approves first test of CRISPR to correct genetic defect causing sickle cell disease

March 30, 2021
By Robert Sanders | UC Berkeley media relations

Sickle cell patients such as Cassandra Trimnell and Evie James Junior and UCSF physician Mark Walters talk about the severe pain experienced by those with the disease and the potential benefits of a CRISPR cure. (Video by UC Berkeley Public Affairs; video of Evie Junior by Colin Weatherby, courtesy UCLA’s Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research)

In 2014, two years after her Nobel Prize-winning invention of CRISPR-Cas9 genome editing, Jennifer Doudna thought the technology was mature enough to tackle a cure for a devastating hereditary...

Nano strategy overcomes barriers to plant genetic engineering

May 28, 2020
by Wallace Ravven | Berkeley Research

Markita Landry files paten for new nanotube technology

Markita Landry and UC Berkeley recently filed patents on a new nanotube technology to delete genes in crop plants without the risk of inserting new genes. Editing the genome of crop plants can boost such traits as disease resistance or drought tolerance. Since the new process adds no genes to the plant genome in the editing process, it conforms to non-GMO requirements in the U.S. and several other countries outside Europe.

'Human Nature': film review

March 16, 2020
John DeFore| Hollywood Reporter

film review of 'Human Nature

How many newspaper stories, magazine features and TV segments have been produced so far that marvel at the revolutionary capabilities of CRISPR while giving almost no idea at all how the gene-editing discovery actually works? Those who lament the state of science journalism should take note of 'Human Nature', in which Adam Bolt and helpful scientists offer an easily understood introduction to techniques often described with head-scratching phrases like "it's a word processor for DNA!" A cogent, wide-ranging look at both the discovery and the nascent, soon-to-be-giant fights humans are having over it, the documentary should be welcomed in its limited theatrical release and will be even more useful on video.

Meet CRISPR: Humanity’s shiny new tool

December 31, 2019
Taylor Beck | California Magazine

CRISPR Cas9

One of biology’s wilder facts is that we’re all family. You and me, sure, but also me and a mushroom. Triceratops shared genes with you. So does the virus that makes you cough, and a rosebush. Bacteria left us on the tree of life around 2.7 billion years ago, but the wet world they came from is still ours: One code runs all of life. The same proteins that imprint memories in your neurons, for example, do so in octopi, ravens, and sea slugs. This genetic conservation means tricks from one species can be hijacked. If you stick a jellyfish gene in a monkey, it’ll glow green.

The first U.S. trials in people put CRISPR to the test in 2019

December 30, 2019
Tina Hesman Saey | Sciencenews.com

first US CRISPR trials

When it was unveiled in 2012, people had great hopes that the gene editor CRISPR/Cas9 could treat or even cure hundreds to thousands of genetic diseases. This year, researchers in the United States began testing the gene editor in people, a crucial first step in determining whether the technology can fulfill its medical promise.

UC receives patent for use of CRISPR-Cas9 to tune gene expression

May 29, 2019
by Public Affairs, UC Berkeley

Jennifer Doudna holds a model of the CRISPR-Cas9 protein (white) interacting with DNA (orange and blue).The U.S. Patent and Trademark Office today issued a patent to the University of California (UC), the University of Vienna and French biologist Emmanuelle Charpentier that covers methods of modulating DNA transcription using the CRISPR-Cas9 system.

Scientists use DNA origami to alter gene expression in plants

April 4, 2019
Marge d'Wylde |College of Chemistry

DNA origami could change the way we alter plants

new research reported from the lab of Markita Landry, a professor of chemical and biomolecular engineering at UC Berkeley, a team of scientists has taken an original approach of using DNA origami nanotechnology to slip through plant cell walls and graft small interfering RNA (siRNA) directly onto plant cells. Their research shows it is possible to directly silence genes in plants without damaging plant tissues, and without making any alterations to the plant’s genome.