CRISPR

CRISPR Cas9 explained. CRISPR (clustered regularly interspaced short palindromic repeats) are segments of prokaryotic DNA containing short repetitions of base sequences. is an RNA-guided gene-editing platform that makes use of a bacterially derived protein (Cas9) and a synthetic guide RNA to introduce a double strand break at a specific location within the genome.

Cas9 is an enzyme that snips DNA, and CRISPR is a collection of DNA sequences that tells Cas9 exactly where to snip.

How to edit the genes of nature’s master manipulators

December 6, 2022
by Aliyah Kovner | Berkeley Lab

Hourglass structure

Scientists are using CRISPR to engineer the viruses that evolved to engineer bacteria. Illustration Davian Ho.

CRISPR, the Nobel Prize-winning gene editing technology, is poised to have a profound impact on the fields of microbiology and medicine yet again.

A team led by CRISPR pioneer...

Reshaping evolution

January 10, 2022
TED Radio Hour | NPR

Illustration of DNA and parade of animals and man

Illustration of DNA with parade of animals and humans. (Adobestock)

New innovations in gene and stem cell technology have the power to shape ecosystems and even change humanity. This hour, TED speakers share the breakthroughs heralding the next scientific revolution.

Guests...

Genetically modified rice could emit fewer greenhouse gases

January 3, 2022
Eric Roston | Bloomberg

Rice planting

Photo: Worker planting rice. (Adobe Stock)

Fifteen years after their initial meeting to discuss what has become the CRISPR revolution, Professors Jill Banfield(link is external) and Jennifer Doudna(link is external) of UC Berkeley,...

Newly-discovered 'Borg' DNA Is unlike anything scientists have ever seen

July 15, 2021
Becky Ferreira | Vice.com

DNA strand

Image: KTSDESIGN/ Science Photot Library via Getty Images. Photo courtesy vice.com.

"Borgs" are extrachromosomal elements, meaning that these DNA sequences are found outside the chromosomes that lie within the nucleus of most cells and that contain the majority of an organism’s genetic material. Examples of extrachromosomal elements include plasmids, which can...

FDA approves first test of CRISPR to correct genetic defect causing sickle cell disease

March 30, 2021
By Robert Sanders | UC Berkeley media relations

Sickle cell patients such as Cassandra Trimnell and Evie James Junior and UCSF physician Mark Walters talk about the severe pain experienced by those with the disease and the potential benefits of a CRISPR cure. (Video by UC Berkeley Public Affairs; video of Evie Junior by Colin Weatherby, courtesy UCLA’s Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research)

In 2014, two years after her Nobel Prize-winning invention of CRISPR-Cas9 genome editing, Jennifer Doudna thought the technology was mature enough to tackle a cure for a devastating hereditary...

Crispr, not just for gene editing

October 30, 2020
Jennifer Newton | Chemistry World

Illustration of Crispr-Cas activity

Crispr–Cas is part of an ancient bacterial immune system that detects and chops up invading viruses’ DNA. Source: © Science Photo Library

Thanks to the 2020(link is external)...

UC Berkeley launches trial of saliva test for COVID-19

June 30, 2020
By Robert Sanders | UC Berkeley media relations

COVID-19 saliva based test

Scientists from the Innovative Genomics Institute (IGI), the same UC Berkeley group that rapidly popped up a state-of-the-art COVID-19 testing laboratory in March, are now trialing a quicker way to obtain patient samples: through saliva. Saliva, collected in the same way companies like 23andMe and Ancestry.com get samples for DNA genealogy analysis, can be gathered without medical supervision, and that saves time, money and precious PPE.

Megaphages harbor mini-Cas proteins ideal for gene editing

July 20, 2020
By Robert Sanders | UC Berkeley media relations

Illustration of a megaphage injecting its DNA into a gene

The DNA-cutting proteins central to CRISPR-Cas9 and related gene-editing tools originally came from bacteria, but a newfound variety of Cas proteins apparently evolved in viruses that infect bacteria. The new Cas proteins were found in the largest known bacteria-infecting viruses, called bacteriophages, and are the most compact working Cas variants yet discovered — half the size of today’s workhorse, Cas9.

Nano strategy overcomes barriers to plant genetic engineering

May 28, 2020
by Wallace Ravven | Berkeley Research

Markita Landry files paten for new nanotube technology

Markita Landry and UC Berkeley recently filed patents on a new nanotube technology to delete genes in crop plants without the risk of inserting new genes. Editing the genome of crop plants can boost such traits as disease resistance or drought tolerance. Since the new process adds no genes to the plant genome in the editing process, it conforms to non-GMO requirements in the U.S. and several other countries outside Europe.

With its coronavirus rapid paper test strip, this CRISPR startup wants to help halt a pandemic

March 16, 2020
John Cumbers _ Forbes.com

new CRISPR based test for coronavirus

A potential solution to speeding up the diagnostics of coronavirus may have presented itself in the form of the gene editing molecular tool called CRISPR. Combined with high-scale advances in automation and computation, CRISPR promises to be a real game-changer in the field of synthetic biology, impacting everything from chemicals and materials to food and health. CRISPR’s precision has an uncanny ability to find a specific sequence within a sample, and one startup has a way to test for coronavirus in 30 minutes (the whole process including sample preparation will take about 4 hours).