CRISPR

CRISPR Cas9 explained. CRISPR (clustered regularly interspaced short palindromic repeats) are segments of prokaryotic DNA containing short repetitions of base sequences. is an RNA-guided gene-editing platform that makes use of a bacterially derived protein (Cas9) and a synthetic guide RNA to introduce a double strand break at a specific location within the genome.

Cas9 is an enzyme that snips DNA, and CRISPR is a collection of DNA sequences that tells Cas9 exactly where to snip.

Crispr fans dream of a populist future for gene editing

August 18, 2017

Jennifer DoudnaOn Wednesday and Thursday (August 16-17, 2017) UC Berkeley welcomed about 300 people—scientists, CEOs, farmers, regulators, conservationists, and interested citizens—to its campus to take a hard look at the wünderenzyme known as Cas9.

Aug. 16-17 CRISPRcon to focus on societal issues of gene editing

August 3, 2017

CRISPRconUC Berkeley is hosting a two-day conference Aug. 16-17 that will bring together farmers, doctors, patients, environmentalists, consumers, nonprofits, community leaders and scientists to discuss potential applications of CRISPR technology, ranging from human and animal health to agriculture and conservation.

In breakthrough, scientists edit a dangerous mutation from genes in human embryos

August 2, 2017

Jennifer Doudna"It feels a bit like a 'one small step for (hu)mans, one giant leap for (hu)mankind' moment," Jennifer Doudna, a biochemist who helped discover the gene-editing method used, called CRISPR-Cas9, said in an email.

"I expect these results will be encouraging to those who hope to...

CRISPR pioneer Jennifer Doudna struggles with the ethical implications of what she has wrought

July 21, 2017

Berkeley professor Jennifer Doudna Jennifer Doudna faces an uncommon challenge for a scientist at the top of her form and the forefront of her field: While she watches her work produce "breathtaking" advances in the understanding of human biology, she's struggling with its...

Researchers discover how CRISPR proteins find their target

July 24, 2017

Jennifer DoudnaThe paper, which will be published online July 20 in Science by Jennifer Doudna and her research group, used electron microscopy and X-ray crystallography, performed at the Advanced Light Source at Lawrence Berkeley National Laboratory, the Stanford Linear Accelerator Center, and the HHMI electron microscope facility at UC Berkeley, to capture structures of Cas1-Cas2 in the act of inserting...

Defense department pours $65 million into making CRISPR safer

July 19, 2017

Jennifer DoudnaWith one eye on potential bioterrorism threats, the Defense Advanced Research Projects Agency today announced $65 million in funding to seven projects around the country – including one led by UC Berkeley – to improve the safety and accuracy of gene editing.

Anti-CRISPR proteins decrease off-target side effects of CRISPR-Cas9

July 12, 2017

Jennifer DoudnaResearch now shows that the countermeasure viruses came up with — inhibitory proteins referred to as anti-CRISPRs — can be used to improve CRISPR-Cas9 as a gene-therapy tool, decreasing off-target gene editing that could cause unwanted side effects.

Book Review: A Crack in Creation

June 17, 2017

A Crack in CreationJennifer Doudna, Crispr and a great scientific breakthrough. An invaluable account, by Doudna and Samuel Sternberg, of their role in the revolution that is genome editing

Summer Reading List: A Crack in Creation

May 2, 2017

A Crack in CreationPart of the 2017 Berkeley Summer Reading List, A Crack in Creation discusses the origin and impact of the CRISPR-Cas9 gene-editing technology co-invented by UC Berkeley biologist Jennifer Doudna. Hailed as the scientific breakthrough of the century,...